cd45 microbeads, human  (Miltenyi Biotec)

Journal: Cells

Article Title: Alterations in Circulating Progenitor Cell Composition in Rheumatoid Arthritis

doi: 10.3390/cells15080726

Figure Lengend Snippet: Peripheral blood cell clusters and their proportions. ( a ) Cells were gated for size with the forward-light scatter versus granularity/complexity side scatter (FSC vs. SSC) and debris, doublets, and dead cells were excluded. Coloured rectangles highlight four cellular quadrants: CD45 − CD31 − (blue), CD45 + CD31 − (red), CD45 + CD31 + (orange), and CD31 + CD45 − (green). ( b ) Average cell frequency in HC (n = 9) and RA (n = 12). Pairwise statistical Chi-test. Cell frequencies of ( c ) CD45 + CD31 − , ( d ) CD45 + CD31 + ( e ) CD45 − CD31 − and ( f ) CD31 + CD45 − cells. Mean and standard deviation (SD); unpaired t -test ( c , d ) and Mann-Whitney test ( e , f ). Unsupervised dimensionality reduction analysis of the measured cell surface proteins in the concatenated file containing healthy controls (HC) (n = 9) and rheumatoid arthritis (RA) (n = 12) with a total of 260.000 life cells is visualised in ( g ) Uniform Manifold Approximation and Projection (UMAP) and ( h ) Triplet-based Manifold Approximation (triMAP) plots. ( i ) Distribution of the populations according to CD45 and CD31 expression. Populations from the Flow Self-Organizing Map (FlowSOM) algorithm are coloured and labelled with numbers from 1 to 20. ( j ) Mean proportions of each coloured and numbered population within the HC (n = 9) and RA (n = 12) cohort. Pairwise statistical Chi-test. ( k ) Heatmap from the FlowSOM cluster analysis shows the expression levels of the markers with intensity levels for each coloured and name-coded cell population (green/yellow panel) and the intensity of each population for the HC (n = 9) and RA (n = 12) (orange/yellow).

Article Snippet: CD45 , FITC-A , 130-113-117 , Miltenyi Biotec , 1/150 , B2.

Techniques: Standard Deviation, MANN-WHITNEY, Expressing

Journal: Cells

Article Title: Alterations in Circulating Progenitor Cell Composition in Rheumatoid Arthritis

doi: 10.3390/cells15080726

Figure Lengend Snippet: CD45 + CD31 int cells were reduced in RA patients. ( a ) CD45/CD31 dot plot showing populations 12, 4 and 13. Pop 4 expressed high levels of ( b ) CD133 and ( c ) CD34 (red dots). Bar graphs show a significantly reduced frequency in the percentage of ( d ) Pop 12 and ( e ) Pop 4 in RA patients (n = 12) when compared to HC (n = 9). Mean and SD; Mann–Whitney and t -test, respectively. ( f ) Heat map showing log fold change differences in PECAM1 expression in peripheral mononuclear cells (PBMCs) (RA n = 5, PsA n = 6) and in immune cells within synovial tissues (RA n = 13, PsA n = 12). MAST test, * p < 0.05, *** p < 0.001. Mean fluorescence intensity (MFI) of CD31 expression on ( g ) naïve CD4 T cells, ( h ) central memory (TCM), ( i ) effector memory (TEM), and ( j ) T cells expressing CD45RA (TEMRA). n = 9 per group, all Mann–Whitney, except in G, the t -test was used. All dot plots show mean and SD.

Article Snippet: CD45 , FITC-A , 130-113-117 , Miltenyi Biotec , 1/150 , B2.

Techniques: MANN-WHITNEY, Expressing, Fluorescence

Journal: Cells

Article Title: Alterations in Circulating Progenitor Cell Composition in Rheumatoid Arthritis

doi: 10.3390/cells15080726

Figure Lengend Snippet: CD235a + and CD45 − CD31 − stromal cells circulating in peripheral blood. ( a ) CD45/CD31 dot plot showing populations 16, 17, 18, 19 and 20. ( b ) UMAP shows the size of each cluster, and ( c ) TriMAP shows the relative cluster location. CD45/CD31 plots highlighting the expression of ( d ) CD235a, ( e ) CD133, ( f ) CD34 and ( g ) CD105. The intensity scale indicates high expression of the marker in red. Frequency of ( h ) Pop 20, ( i ) Pop 17, and ( j ) Pop 18. Mann-Whitney test. Dot plots showing the expression of ( k ) CD34/CD133, ( l ) CD271/ CD105, and CD90 with ( m ) CD105, ( n ) CD271, and ( o ) PDPN in Pop 16 and Pop 19. Frequency of ( p ) Pop 16 and ( q ) Pop 19. t -test for Pop 16; Mann–Whitney test for Pop 19. All dot plots show mean and SD, n = 12 RA and 9 HC.

Article Snippet: CD45 , FITC-A , 130-113-117 , Miltenyi Biotec , 1/150 , B2.

Techniques: Expressing, Marker, MANN-WHITNEY